RNA'Protein Interaction Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edition: | 1st ed. 1999. |
| Series: | Methods in Molecular Biology,
118 |
| Subjects: |
Table of Contents:
- Labeling and Purification of RNA Synthesized by In Vitro Transcription
- Joining RNA Molecules with T4 DNA Ligase
- RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs
- The Methylene Blue Mediated Photocrosslinking Method for Dectection of Proteins that Interact with Double-Stranded RNA
- Probing RNA-Protein Interactions by Psoralen Photocrosslinking
- Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI-MS
- RNA Footprinting and Modification Interference Analysis
- Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes
- Nitrocellulose Filter Binding for Determination of Dissociation Constants
- Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays
- PACE Analysis of RNA-Peptide Interactions
- Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance
- An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins
- Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay
- In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display
- In Vitro Selection of Aptamers from RNA Libraries
- Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences
- Northwestern Screening of Expression Libraries
- Screening Expression Libraries with Solution-Based Assay
- An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes
- Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
- Purification of U Small Nuclear Ribonucleoprotein Particles
- Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes
- Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
- Mammalian In Vitro Splicing Assays
- Yeast Pre-mRNA Splicing Extracts
- Prespliceosome and Spliceosome Isolation and Analysis
- A Yeast Spliceosome Assay
- Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing
- In Vivo SELEX in Vertebrate Cells
- Purification of SR Protein Splicing Factors
- Processing mRNA 3' Ends In Vitro
- Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
- In Vitro Translation Extracts from Tissue Culture Cells
- Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes.