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130517s2013 xxu| s |||| 0|eng d |
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|a 9781627034012
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| 024 |
7 |
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|a 10.1007/978-1-62703-401-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Plant Lipid Signaling Protocols /
|c edited by Teun Munnik, Ingo Heilmann.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XI, 305 p. 37 illus., 15 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1009
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| 505 |
0 |
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|a Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC -- Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC -- Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography -- Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay -- Quantification of Diacylglycerol by Mass Spectrometry -- Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK -- Use of Phospholipase A2 for the Production of Lysophospholipids -- Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography -- Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry -- Detection and Quantification of Plant Sphingolipids by LC-MS -- Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid -- Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants -- Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR -- Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides -- Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy -- kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy.
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| 650 |
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0 |
|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Munnik, Teun.
|e editor.
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| 700 |
1 |
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|a Heilmann, Ingo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|