Plant Lipid Signaling Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
1009 |
| Materias: |
Tabla de Contenidos:
- Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC
- Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC
- Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography
- Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay
- Quantification of Diacylglycerol by Mass Spectrometry
- Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK
- Use of Phospholipase A2 for the Production of Lysophospholipids
- Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography
- Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry
- Detection and Quantification of Plant Sphingolipids by LC-MS
- Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid
- Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants
- Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR
- Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides
- Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays
- Assaying Inositol and Phosphoinositide Phosphatase Enzymes
- Determination of Phospholipase C Activity In Vitro
- Assaying Non-Specific Phospholipase C (NPC) Activity
- Assaying Different Types of Plant Phospholipase D Activities In Vitro
- Measuring PLD Activity In Vivo
- Assaying Plant Phosphatidic Acid Phosphatase Activity
- Assay of Phospholipase A Activity
- Lipid-Binding Analysis using a Fat Blot Assay
- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions
- Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties
- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants
- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy
- kinase Assays
- Assaying Inositol and Phosphoinositide Phosphatase Enzymes
- Determination of Phospholipase C Activity In Vitro
- Assaying Non-Specific Phospholipase C (NPC) Activity
- Assaying Different Types of Plant Phospholipase D Activities In Vitro
- Measuring PLD Activity In Vivo
- Assaying Plant Phosphatidic Acid Phosphatase Activity
- Assay of Phospholipase A Activity
- Lipid-Binding Analysis using a Fat Blot Assay
- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions
- Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties
- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants
- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy.