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120917s2013 xxu| s |||| 0|eng d |
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|a 9781627030892
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| 024 |
7 |
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|a 10.1007/978-1-62703-089-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Phytoplasma :
|b Methods and Protocols /
|c edited by Matt Dickinson, Jennifer Hodgetts.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XIII, 421 p. 68 illus., 45 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 938
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| 505 |
0 |
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|a The Phytoplasmas - An Introduction -- Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus -- Micropropagation and Maintenance of Phytoplasmas in Tissue Culture -- Dodder Transmission of Phytoplasmas -- Insect Maintenance and Transmission -- Capturing Insect Vectors of Phytoplasmas -- Insect Vector Transmission Assays -- Molecular Identification of Phytoplasma Vector Species -- Dienes’ Staining and Light Microscopy for Phytoplasma Visualization -- DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas -- Visualisation of Phytoplasmas using Electron Microscopy -- Automated DNA Extraction for Large Numbers of Plant Samples -- DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts -- Nested PCR and RFLP Analysis Based on the 16S rRNA Gene -- PCR and RFLP Analyses Based on the Ribosomal Protein Operon -- Tuf and secY PCR Amplification and Genotyping of Phytoplasmas -- PCR Analysis of Phytoplasmas Based on the secA Gene -- Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains -- Microarrays for Universal Detection and Identification of Phytoplasmas -- T-RFLP for Detection and Identification of Phytoplasmas in Plants -- Real-Time PCR for Universal Phytoplasma Detection and Quantification -- A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA -- Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group -- Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions -- In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP) -- DNA Bar-Coding for Phytoplasma Identification -- Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison -- The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment -- Phytoplasma Proteomic Analysis -- Preparation of Phytoplasma Membrane Recombinant Proteins -- Phytoplasma Plasmid DNA Extraction -- Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA -- Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants -- Mapping the Phytoplasma Chromosome.
|
| 650 |
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0 |
|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
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0 |
|a Plant genetics.
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| 650 |
1 |
4 |
|a Plant Sciences.
|
| 650 |
2 |
4 |
|a Plant Genetics and Genomics.
|
| 700 |
1 |
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|a Dickinson, Matt.
|e editor.
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| 700 |
1 |
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|a Hodgetts, Jennifer.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|