Phytoplasma : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Dickinson, Matt. (Editor ), Hodgetts, Jennifer. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 938
Materias:
Tabla de Contenidos:
  • The Phytoplasmas - An Introduction
  • Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus
  • Micropropagation and Maintenance of Phytoplasmas in Tissue Culture
  • Dodder Transmission of Phytoplasmas
  • Insect Maintenance and Transmission
  • Capturing Insect Vectors of Phytoplasmas
  • Insect Vector Transmission Assays
  • Molecular Identification of Phytoplasma Vector Species
  • Dienes’ Staining and Light Microscopy for Phytoplasma Visualization
  • DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas
  • Visualisation of Phytoplasmas using Electron Microscopy
  • Automated DNA Extraction for Large Numbers of Plant Samples
  • DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts
  • Nested PCR and RFLP Analysis Based on the 16S rRNA Gene
  • PCR and RFLP Analyses Based on the Ribosomal Protein Operon
  • Tuf and secY PCR Amplification and Genotyping of Phytoplasmas
  • PCR Analysis of Phytoplasmas Based on the secA Gene
  • Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains
  • Microarrays for Universal Detection and Identification of Phytoplasmas
  • T-RFLP for Detection and Identification of Phytoplasmas in Plants
  • Real-Time PCR for Universal Phytoplasma Detection and Quantification
  • A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA
  • Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group
  • Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions
  • In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)
  • DNA Bar-Coding for Phytoplasma Identification
  • Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison
  • The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment
  • Phytoplasma Proteomic Analysis
  • Preparation of Phytoplasma Membrane Recombinant Proteins
  • Phytoplasma Plasmid DNA Extraction
  • Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA
  • Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants
  • Mapping the Phytoplasma Chromosome.