Rat Genomics : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edición: | 1st ed. 2010. |
| Colección: | Methods in Molecular Biology,
597 |
| Materias: |
Tabla de Contenidos:
- The Rat: A Model Used in Biomedical Research
- Genetic Mapping and Positional Cloning
- Sequencing of the Rat Genome and Databases
- Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators)
- Inducible and Conditional Promoter Systems to Generate Transgenic Animals
- Generation of Transgenic Rats by Microinjection of Short DNA Fragments
- Generation of Transgenic Rats Using YAC and BAC DNA Constructs
- The Use of Lentiviral Vectors to Obtain Transgenic Rats
- Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA
- Procedures for Somatic Cell Nuclear Transfer in the Rat
- ENU Mutagenesis to Generate Genetically Modified Rat Models
- Establishment of Embryonic Stem Cells from Rat Blastocysts
- Derivation, Culture, and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts
- Interference RNA for In vivo Knock-Down of Gene Expression or Genome-Wide Screening Using shRNA
- Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases
- Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics
- Generation of Congenic and Consomic Rat Strains
- Generation of Rat “Supersonic” Congenic/Conplastic Strains Using Superovulation and Embryo Transfer
- Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms
- A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites
- Cryopreservation and Orthotopic Transplantation of Rat Ovaries
- Techniques for In Vitro and In Vivo Fertilization in the Rat
- Rat Strain Repositories
- Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases
- Rat Genomics Applied to Psychiatric Research
- Genomics Studies of Immune-Mediated Diseases Using the BN–LEW Rat Model
- Rat Models of Cardiovascular Diseases
- Use of Rat Genomics for Investigating the Metabolic Syndrome
- Genomic Research in Rat Models of Kidney Disease
- Cancer Research in Rat Models.