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140206s2014 xxu| s |||| 0|eng d |
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|a 9781627038454
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| 024 |
7 |
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|a 10.1007/978-1-62703-845-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Neutrophil Methods and Protocols /
|c edited by Mark T. Quinn, Frank R. DeLeo.
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| 250 |
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|a 2nd ed. 2014.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XVI, 551 p. 93 illus., 28 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1124
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| 505 |
0 |
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|a The Role of Neutrophils in the Immune System: An Overview -- Isolation of Human Neutrophils -- Neutrophil Isolation from Non-Human Species -- Collection of In vivo Transmigrated Neutrophils from Human Skin -- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers -- Rho Family and Rap GTPase Activation Assays -- Measurement of Phospholipid Metabolism in Intact Neutrophils -- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils -- Analysis of Electrophysiological Properties and Responses of Neutrophils -- Analysis of Neutrophil Apoptosis -- Microinjection Methods for Neutrophils -- Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells -- Neutrophil Migration Through Extracellular Matrix -- Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish -- Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion -- Immunofluorescence and Confocal Microscopy of Neutrophils -- Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils -- Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry -- Analysis of Neutrophil Bactericidal Activity -- Induction and Quantification of Neutrophil Extracellular Traps -- Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes -- Cell-free NADPH Oxidase Assays: “In vitro Veritas” -- Assessment of Priming of the Human Neutrophil Respiratory Burst -- Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane -- Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies -- Genome-Scale Transcript Analyses with Human Neutrophils -- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils -- High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis -- Detection of Intact Transcription Factors in Human Neutrophils -- Disorders of Neutrophil Function: An Overview -- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders -- Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency.
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| 650 |
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0 |
|a Infectious diseases.
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| 650 |
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0 |
|a Microbial genetics.
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| 650 |
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0 |
|a Microbial genomics.
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| 650 |
1 |
4 |
|a Infectious Diseases.
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| 650 |
2 |
4 |
|a Microbial Genetics and Genomics.
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| 700 |
1 |
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|a Quinn, Mark T.
|e editor.
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| 700 |
1 |
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|a DeLeo, Frank R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|