Neutrophil Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Quinn, Mark T. (Editor ), DeLeo, Frank R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:2nd ed. 2014.
Colección:Methods in Molecular Biology, 1124
Materias:
Tabla de Contenidos:
  • The Role of Neutrophils in the Immune System: An Overview
  • Isolation of Human Neutrophils
  • Neutrophil Isolation from Non-Human Species
  • Collection of In vivo Transmigrated Neutrophils from Human Skin
  • Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
  • Rho Family and Rap GTPase Activation Assays
  • Measurement of Phospholipid Metabolism in Intact Neutrophils
  • Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils
  • Analysis of Electrophysiological Properties and Responses of Neutrophils
  • Analysis of Neutrophil Apoptosis
  • Microinjection Methods for Neutrophils
  • Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells
  • Neutrophil Migration Through Extracellular Matrix
  • Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish
  • Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion
  • Immunofluorescence and Confocal Microscopy of Neutrophils
  • Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils
  • Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry
  • Analysis of Neutrophil Bactericidal Activity
  • Induction and Quantification of Neutrophil Extracellular Traps
  • Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes
  • Cell-free NADPH Oxidase Assays: “In vitro Veritas”
  • Assessment of Priming of the Human Neutrophil Respiratory Burst
  • Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane
  • Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies
  • Genome-Scale Transcript Analyses with Human Neutrophils
  • Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils
  • High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis
  • Detection of Intact Transcription Factors in Human Neutrophils
  • Disorders of Neutrophil Function: An Overview
  • Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
  • Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency.