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04316nam a22003015i 4500 |
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150328s2015 xxu| s |||| 0|eng d |
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|a 9781493925506
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024 |
7 |
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|a 10.1007/978-1-4939-2550-6
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Proteomic Profiling :
|b Methods and Protocols /
|c edited by Anton Posch.
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250 |
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|a 1st ed. 2015.
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260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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300 |
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|a XV, 501 p. 119 illus., 68 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1295
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|a Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization -- Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples -- Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting -- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling -- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model -- Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions -- A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain -- Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses -- Dynamic Range Compression with ProteoMiner: Principles and Examples -- Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue -- Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics -- Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry -- P Isolation of Extracellular Vesicles for Proteomic Profiling -- A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods -- Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes -- Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method -- Step by Step Preparation of Proteins for Mass Spectrometric Analysis -- Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry -- Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS -- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation -- Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing -- Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis -- Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation -- Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry -- In-Gel Peptide IEF Sample Preparation for LC/MS Analysis -- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology -- 2-D Western Blotting for Evaluation of Antibodies Developed for Detection of Host Cell Protein -- Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes -- Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes -- A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin -- Identification of SUMO E3 Ligase Specific Substrates Using the HuProt Human Proteome Microarray -- Amyloid-Binding Proteins: Affinity Based Separation, Proteomic Identification and Optical Biosensor Validation -- Proteomic Profiling by Nanomaterials-Based Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for High-Resolution Data and Novel Protein Information Directly from Biological Samples.
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650 |
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|a Proteins .
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|a Proteomics.
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|a Protein Science.
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650 |
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|a Proteomics.
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700 |
1 |
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|a Posch, Anton.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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