Proteomic Profiling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1295 |
| Materias: |
Tabla de Contenidos:
- Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization
- Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples
- Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting
- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling
- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model
- Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions
- A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain
- Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses
- Dynamic Range Compression with ProteoMiner: Principles and Examples
- Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
- Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics
- Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry
- P Isolation of Extracellular Vesicles for Proteomic Profiling
- A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods
- Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes
- Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method
- Step by Step Preparation of Proteins for Mass Spectrometric Analysis
- Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry
- Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS
- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation
- Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing
- Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis
- Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation
- Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry
- In-Gel Peptide IEF Sample Preparation for LC/MS Analysis
- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology
- 2-D Western Blotting for Evaluation of Antibodies Developed for Detection of Host Cell Protein
- Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes
- Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes
- A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin
- Identification of SUMO E3 Ligase Specific Substrates Using the HuProt Human Proteome Microarray
- Amyloid-Binding Proteins: Affinity Based Separation, Proteomic Identification and Optical Biosensor Validation
- Proteomic Profiling by Nanomaterials-Based Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for High-Resolution Data and Novel Protein Information Directly from Biological Samples.