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170921s2017 xxu| s |||| 0|eng d |
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|a 9781493972869
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| 024 |
7 |
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|a 10.1007/978-1-4939-7286-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Plant Germline Development :
|b Methods and Protocols /
|c edited by Anja Schmidt.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XIII, 422 p. 103 illus., 86 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1669
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| 505 |
0 |
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|a An Introduction to Male Germline Development -- Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants -- 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice -- Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana -- Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers -- Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy -- In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes -- Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins -- Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes -- Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy -- Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures -- Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues -- Whole Mount RNA-FISH on Ovules and Developing Seeds -- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy -- Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes -- Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS) -- Isolation of Rice Sperm Cells for Transcriptional Profiling -- Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation -- Isolation and Detection of Small RNAs from Pollen -- Analysis of Proteins Enriched in Rice Gamete -- Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract -- Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants -- RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis -- RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data -- Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality -- Agrobacterium-Mediated Sorghum Transformation -- Use of the Cas9 Orthologues from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana -- Transgenic Reproductive Cell Ablation -- In Vivo Reporters for Protein Half-Life -- Conditional Modulation of Biological Processes by Low-Temperature Degrons.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Schmidt, Anja.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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