Plant Germline Development : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1669 |
| Materias: |
Tabla de Contenidos:
- An Introduction to Male Germline Development
- Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants
- 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice
- Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana
- Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers
- Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy
- In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes
- Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins
- Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes
- Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy
- Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures
- Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues
- Whole Mount RNA-FISH on Ovules and Developing Seeds
- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
- Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
- Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)
- Isolation of Rice Sperm Cells for Transcriptional Profiling
- Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation
- Isolation and Detection of Small RNAs from Pollen
- Analysis of Proteins Enriched in Rice Gamete
- Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract
- Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants
- RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis
- RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data
- Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
- Agrobacterium-Mediated Sorghum Transformation
- Use of the Cas9 Orthologues from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana
- Transgenic Reproductive Cell Ablation
- In Vivo Reporters for Protein Half-Life
- Conditional Modulation of Biological Processes by Low-Temperature Degrons.