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20210603145017.0 |
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cr nn 008mamaa |
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110523s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617791260
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| 024 |
7 |
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|a 10.1007/978-1-61779-126-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Receptor Signal Transduction Protocols :
|b Third Edition /
|c edited by Gary B. Willars, R.A. John Challiss.
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| 250 |
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|a 3rd ed. 2011.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
|
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|a XI, 479 p. 84 illus. :
|b online resource.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 746
|
| 505 |
0 |
|
|a Inducible Expression of G Protein-Coupled Receptors in Transfected Cells -- Using the Flp-InTM T-RexTM System to Regulate GPCR Expression -- Viral Infection for GPCR Expression in Eukaryotic Cells -- Generation of Epitope-Tagged GPCRs -- The Use of Site-Directed Mutagenesis to Study GPCRs -- Approaches to Study GPCR Regulation in Native Systems -- Heterologous Expression of GPCRs in Fission Yeast -- Radioligand Binding Methods for Membrane Preparations and Intact Cells -- Quantification of GPCR mRNA Using Real-Time RT-PCR -- Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data -- Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells -- Examining Site-Specific GPCR Phosphorylation -- Ubiquitination of GPCRs -- [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs -- Using Calcium Imaging as a Readout of GPCR Activation -- Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors -- Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins -- The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events -- Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Study of GPCR-Protein Interactions by BRET -- Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization -- Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes -- Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes -- Identification of GPCR Localization in Detergent Resistant Membranes -- Analysis of GPCR Localization and Trafficking -- Statistical Methods in Research.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Willars, Gary B.
|e editor.
|
| 700 |
1 |
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|a Challiss, R.A. John.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|