Receptor Signal Transduction Protocols : Third Edition /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edición: | 3rd ed. 2011. |
| Colección: | Methods in Molecular Biology,
746 |
| Materias: |
Tabla de Contenidos:
- Inducible Expression of G Protein-Coupled Receptors in Transfected Cells
- Using the Flp-InTM T-RexTM System to Regulate GPCR Expression
- Viral Infection for GPCR Expression in Eukaryotic Cells
- Generation of Epitope-Tagged GPCRs
- The Use of Site-Directed Mutagenesis to Study GPCRs
- Approaches to Study GPCR Regulation in Native Systems
- Heterologous Expression of GPCRs in Fission Yeast
- Radioligand Binding Methods for Membrane Preparations and Intact Cells
- Quantification of GPCR mRNA Using Real-Time RT-PCR
- Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data
- Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells
- Examining Site-Specific GPCR Phosphorylation
- Ubiquitination of GPCRs
- [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs
- Using Calcium Imaging as a Readout of GPCR Activation
- Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors
- Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins
- The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events
- Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs
- Study of GPCR-Protein Interactions by BRET
- Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization
- Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes
- Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes
- Identification of GPCR Localization in Detergent Resistant Membranes
- Analysis of GPCR Localization and Trafficking
- Statistical Methods in Research.