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02218nam a22003375i 4500 |
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000291030 |
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20200921133834.0 |
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cr nn 008mamaa |
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190911s2020 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493998371
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| 024 |
7 |
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|a 10.1007/978-1-4939-9837-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Transgenic Mouse :
|b Methods and Protocols /
|c edited by Melissa A. Larson.
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| 250 |
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|a 1st ed. 2020.
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| 260 |
# |
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|a New York, NY :
|b Springer US :
|b Imprint: Humana,
|c 2020.
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| 300 |
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|a X, 236 p. 45 illus., 27 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 2066
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| 505 |
0 |
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|a The History of Transgenesis -- Pronuclear Microinjection of One-Cell Embryos -- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection -- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos -- Targeted Mutations in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device -- Nuclear Transfer and Cloning -- Sperm-Mediated Genetic Modifications -- Genotyping Genetically Modified (GM) Mice -- Nomenclature: Naming Your Gene-Modified Mouse -- Breeding Strategies for Genetically Modified Mice -- Strategies for Behaviorally Phenotyping the Transgenic Mouse -- Cryobanking and Recovery of Genetically Modified Mice -- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques -- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.
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| 650 |
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|a Human genetics.
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| 650 |
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0 |
|a Animal models in research.
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| 650 |
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|a Stem cells.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Animal Models.
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| 650 |
2 |
4 |
|a Stem Cells.
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| 700 |
1 |
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|a Larson, Melissa A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|