Transgenic Mouse : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer US : Imprint: Humana,
2020.
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| Edición: | 1st ed. 2020. |
| Colección: | Methods in Molecular Biology,
2066 |
| Materias: |
Tabla de Contenidos:
- The History of Transgenesis
- Pronuclear Microinjection of One-Cell Embryos
- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection
- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos
- Targeted Mutations in the Mouse via Embryonic Stem Cells
- Blastocyst Microinjection with Embryonic Stem Cells
- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos
- Embryo Transfer Surgery
- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device
- Nuclear Transfer and Cloning
- Sperm-Mediated Genetic Modifications
- Genotyping Genetically Modified (GM) Mice
- Nomenclature: Naming Your Gene-Modified Mouse
- Breeding Strategies for Genetically Modified Mice
- Strategies for Behaviorally Phenotyping the Transgenic Mouse
- Cryobanking and Recovery of Genetically Modified Mice
- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques
- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.