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181218s2019 xxu| s |||| 0|eng d |
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|a 9781493989102
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| 024 |
7 |
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|a 10.1007/978-1-4939-8910-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Hippo Pathway :
|b Methods and Protocols /
|c edited by Alexander Hergovich.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XIV, 374 p. 72 illus., 57 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1893
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| 505 |
0 |
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|a The Power of Drosophila Genetics: The Discovery of the Hippo Pathway -- Drosophila Genetics: The Power of Genetic Mosaic Approaches -- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues -- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs -- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence -- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions -- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells -- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells -- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors -- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells -- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells -- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays -- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids -- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview -- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels -- Hippo Pathway Regulation by Tyrosine Kinases -- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway -- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro -- GST Pull-Down Assay to Measure Complex Formations -- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag -- Quantifying the Kinase Activities of MST1/2 -- Measuring the Kinase Activities of the LATS/NDR Protein Kinases -- MST1/2 Kinase Assays Using Recombinant Proteins -- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development -- The Hippo Signaling Pathway in Regenerative Medicine.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Hergovich, Alexander.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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