The Hippo Pathway : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1893 |
| Materias: |
Tabla de Contenidos:
- The Power of Drosophila Genetics: The Discovery of the Hippo Pathway
- Drosophila Genetics: The Power of Genetic Mosaic Approaches
- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues
- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs
- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence
- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions
- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells
- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells
- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors
- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells
- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells
- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays
- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids
- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview
- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels
- Hippo Pathway Regulation by Tyrosine Kinases
- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway
- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro
- GST Pull-Down Assay to Measure Complex Formations
- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag
- Quantifying the Kinase Activities of MST1/2
- Measuring the Kinase Activities of the LATS/NDR Protein Kinases
- MST1/2 Kinase Assays Using Recombinant Proteins
- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development
- The Hippo Signaling Pathway in Regenerative Medicine.