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02374nam a22003015i 4500 |
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20210920203855.0 |
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151020s2016 xxu| s |||| 0|eng d |
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|a 9781493931583
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| 024 |
7 |
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|a 10.1007/978-1-4939-3158-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Genetic Manipulation of Staphylococci :
|b Methods and Protocols /
|c edited by Jeffrey L. Bose.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a X, 186 p. 23 illus., 7 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1373
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| 505 |
0 |
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|a Understanding Staphylococcal Nomenclature -- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus -- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci -- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning -- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products -- Method for Preparation and Electroporation of S. aureus and S. epidermidis -- Rapid Isolation of DNA from Staphylococcus -- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method -- Bacteriophage Transduction in Staphylococcus aureus -- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus -- Conjugative Transfer in Staphylococcus aureus -- Allelic Exchange -- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917 -- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis -- Chemical and UV Mutagenesis -- Pulsed Field Gel Electrophoresis -- RNA-Sequencing of Staphylococcus aureus Messenger RNA -- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression -- Electrophoretic Mobility Shift Assays -- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.
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| 650 |
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0 |
|a Microbiology.
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| 650 |
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0 |
|a Gene expression.
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| 650 |
1 |
4 |
|a Microbiology.
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| 650 |
2 |
4 |
|a Gene Expression.
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| 700 |
1 |
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|a Bose, Jeffrey L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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