The Genetic Manipulation of Staphylococci : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Methods in Molecular Biology,
1373 |
| Materias: |
Tabla de Contenidos:
- Understanding Staphylococcal Nomenclature
- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus
- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci
- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning
- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
- Method for Preparation and Electroporation of S. aureus and S. epidermidis
- Rapid Isolation of DNA from Staphylococcus
- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method
- Bacteriophage Transduction in Staphylococcus aureus
- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
- Conjugative Transfer in Staphylococcus aureus
- Allelic Exchange
- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917
- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis
- Chemical and UV Mutagenesis
- Pulsed Field Gel Electrophoresis
- RNA-Sequencing of Staphylococcus aureus Messenger RNA
- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression
- Electrophoretic Mobility Shift Assays
- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.