The Golgi Complex : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Brown, William J. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:1st ed. 2016.
Series:Methods in Molecular Biology, 1496
Subjects:
Table of Contents:
  • 4D Confocal Imaging of Yeast Organelles
  • Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
  • Imaging Golgi Outposts in Fixed and Living Neurons
  • Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
  • STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
  • Reconstitution of COPI Vesicle and Tubule Formation
  • Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules
  • Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures
  • High Content Analysis of the Golgi Complex by Correlative Screening Microscopy
  • Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo
  • In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures
  • Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function
  • Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway
  • Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle
  • The Role of Lysophospholipid Acyltransferases in the Golgi Complex
  • Methods to Purify and Assay Secretory Pathway Kinases.