Therapeutic Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Voynov, Vladimir. (Editor ), Caravella, Justin A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:2nd ed. 2012.
Colección:Methods in Molecular Biology, 899
Materias:
Tabla de Contenidos:
  • Therapeutic Proteins
  • Synthetic Antibody Libraries
  • The Construction of ‘Phylomer’ Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions
  • Ribosome Display and Screening for Protein Therapeutics
  • Yeast Display of Engineered Antibody Domains
  • Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains
  • Engineering of Affibody Molecules for Therapy and Diagnostics
  • Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination
  • Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting
  • Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris
  • Use of E. coli for the Production of a Single Protein
  • Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli
  • Transient Expression Technologies: Past, Present, and Future
  • Stable Transfection Pools for Large Quantity of Protein Production
  • Mammalian Stable Expression of Biotherapeutics
  • Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed
  • Methods for Chromatographic Removal of Endotoxin
  • Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps
  • High-Throughput Quantitative N-Glycan Analysis of Glycoproteins
  • High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris
  • Databases and Tools in Glycobiology
  • Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS
  • Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry
  • Monitoring of Subvisible Particles in Therapeutic Proteins
  • Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms
  • Computational Methods to Predict Therapeutic Protein Aggregation
  • Coarse-Grained Simulations of Protein Aggregation
  • Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins
  • Challenges in the Development and Manufacturing of Antibody-Drug Conjugates.