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180531s2018 xxu| s |||| 0|eng d |
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|a 9781493978717
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| 024 |
7 |
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|a 10.1007/978-1-4939-7871-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Two-Hybrid Systems :
|b Methods and Protocols /
|c edited by Luis Oñate-Sánchez.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XII, 355 p. 58 illus., 50 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1794
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| 505 |
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|a Protein Interactomics by Two-Hybrid Methods -- Making the Right Choice: Critical Parameters of the Y2H Systems -- Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening -- Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System -- The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions -- A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning -- Analyses of Protein Interaction Networks Using Computational Tools -- Options and Considerations When Using a Yeast One-Hybrid System -- Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins -- Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens -- Transcription Factor-Centered Yeast One-Hybrid Assay -- The Yeast Three-Hybrid System for Protein Interactions -- The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants -- Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules -- Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System -- Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells -- Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology -- KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions -- Coimmunoprecipitation of Interacting Proteins in Plants -- Analysis of Protein-Protein Interaction by Co-IP in Human Cells -- Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures -- Transient Transactivation Studies in Nicotiana benthamiana Leaves -- The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions -- Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.
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| 650 |
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|a Proteins .
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| 650 |
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4 |
|a Protein Science.
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| 700 |
1 |
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|a Oñate-Sánchez, Luis.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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