Two-Hybrid Systems : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Oñate-Sánchez, Luis. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1794
Materias:
Tabla de Contenidos:
  • Protein Interactomics by Two-Hybrid Methods
  • Making the Right Choice: Critical Parameters of the Y2H Systems
  • Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening
  • Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System
  • The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions
  • A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning
  • Analyses of Protein Interaction Networks Using Computational Tools
  • Options and Considerations When Using a Yeast One-Hybrid System
  • Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
  • Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens
  • Transcription Factor-Centered Yeast One-Hybrid Assay
  • The Yeast Three-Hybrid System for Protein Interactions
  • The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants
  • Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules
  • Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System
  • Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells
  • Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology
  • KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions
  • Coimmunoprecipitation of Interacting Proteins in Plants
  • Analysis of Protein-Protein Interaction by Co-IP in Human Cells
  • Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures
  • Transient Transactivation Studies in Nicotiana benthamiana Leaves
  • The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions
  • Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.