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|a 9781592596829
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| 024 |
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|a 10.1385/1592596827
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a T Cell Protocols :
|b Development and Activation /
|c edited by Kelly P. Kearse.
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| 250 |
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|a 1st ed. 2000.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2000.
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| 300 |
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|a XIV, 365 p. 267 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 134
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| 505 |
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|a Overview: For Chapters 2 – 15 -- Insights into T-Cell Development Using Transgenic and Knockout Mice -- T Cell Development -- Isolation and Characterization of Murine Early Intrathymic Precursor Populations -- Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture -- Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody -- Transfection and Transcription of Genes in Developing Thymocytes -- Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay -- Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses -- Quantitative Analysis of the Usage of Human T-Cell Receptor ? and ? Chain Variable Regions by Reverse Dot Blot Hybridization -- Flow Cytometric Detection of Cytoplasmic and Surface CD3-? Expression in Developing T Cells -- Assessing Apoptosis of Developing T Cells by Flow Cytometry -- Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS) -- Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning -- Flow Cytometric Analysis of Murine T Lymphocytes -- Production and Characterization of Immature Murine T-Lymphoma Cell Lines -- Production and Characterization of T-Cell Hybridomas -- Overview for Chapters 17–29 -- Something Happens; A Brief History of TCR Signal Transduction -- T-Cell Activation -- Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes -- Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation -- Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry -- Digital Image Analysis of Lymphocyte Activation -- Induction of T-Cell Signaling by Immobilized Integrin Ligands -- Cytotoxic T-Cell Adherence Assay (CAA) -- Detecting Ubiquitinated T-Cell Antigen Receptor Subunits by Immunoblotting -- Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes -- Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry -- Biochemical Analysis of Activated T Lymphocytes -- Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement -- Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4+ T Cells -- Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA).
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
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|a Kearse, Kelly P.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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