T Cell Protocols : Development and Activation /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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| Edición: | 1st ed. 2000. |
| Colección: | Methods in Molecular Biology,
134 |
| Materias: |
Tabla de Contenidos:
- Overview: For Chapters 2 – 15
- Insights into T-Cell Development Using Transgenic and Knockout Mice
- T Cell Development
- Isolation and Characterization of Murine Early Intrathymic Precursor Populations
- Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture
- Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
- Transfection and Transcription of Genes in Developing Thymocytes
- Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay
- Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses
- Quantitative Analysis of the Usage of Human T-Cell Receptor ? and ? Chain Variable Regions by Reverse Dot Blot Hybridization
- Flow Cytometric Detection of Cytoplasmic and Surface CD3-? Expression in Developing T Cells
- Assessing Apoptosis of Developing T Cells by Flow Cytometry
- Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS)
- Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning
- Flow Cytometric Analysis of Murine T Lymphocytes
- Production and Characterization of Immature Murine T-Lymphoma Cell Lines
- Production and Characterization of T-Cell Hybridomas
- Overview for Chapters 17–29
- Something Happens; A Brief History of TCR Signal Transduction
- T-Cell Activation
- Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes
- Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation
- Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry
- Digital Image Analysis of Lymphocyte Activation
- Induction of T-Cell Signaling by Immobilized Integrin Ligands
- Cytotoxic T-Cell Adherence Assay (CAA)
- Detecting Ubiquitinated T-Cell Antigen Receptor Subunits by Immunoblotting
- Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes
- Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry
- Biochemical Analysis of Activated T Lymphocytes
- Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement
- Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4+ T Cells
- Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA).