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160504s2016 xxu| s |||| 0|eng d |
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|a 9781493935307
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| 024 |
7 |
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|a 10.1007/978-1-4939-3530-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Nuclear Envelope :
|b Methods and Protocols /
|c edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XIV, 523 p. 103 illus., 77 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1411
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| 505 |
0 |
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|a Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome -- Exploring the Protein Composition of the Plant Nuclear Envelope -- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes -- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins -- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) -- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP -- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro -- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID -- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners -- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components -- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation -- Analysis of Meiotic Telomere Behavior in the Mouse -- Identification and Validation of Putative Nesprin Variants -- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting -- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties -- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling -- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts -- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells -- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila -- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins -- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion -- DamID Analysis of Nuclear Organization in Caenorhabditis elegans -- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells -- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization -- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System -- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina -- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex -- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane -- Nuclear Protein Transport in Digitonin Permeabilized Cells -- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells -- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Shackleton, Sue.
|e editor.
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| 700 |
1 |
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|a Collas, Philippe.
|e editor.
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| 700 |
1 |
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|a Schirmer, Eric C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|