The Nuclear Envelope : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Methods in Molecular Biology,
1411 |
| Materias: |
Tabla de Contenidos:
- Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome
- Exploring the Protein Composition of the Plant Nuclear Envelope
- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes
- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins
- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)
- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP
- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro
- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID
- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners
- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components
- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation
- Analysis of Meiotic Telomere Behavior in the Mouse
- Identification and Validation of Putative Nesprin Variants
- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting
- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties
- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling
- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts
- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells
- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila
- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins
- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion
- DamID Analysis of Nuclear Organization in Caenorhabditis elegans
- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells
- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization
- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System
- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina
- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex
- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane
- Nuclear Protein Transport in Digitonin Permeabilized Cells
- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells
- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.