Rhodopsin : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Jastrzebska, Beata. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1271
Materias:
Tabla de Contenidos:
  • The G Protein-Coupled Receptor Rhodopsin: A Historical Perspective
  • Rhodopsin Purification from Dark Adapted Bovine Retina
  • Mammalian Expression, Purification, and Crystallization of Rhodopsin Variants
  • Imaging of Rhodopsin Crystals with Two-Photon Microscopy
  • Functional Stability of Rhodopsin in a Bicelle System: Evaluating G-Protein Activation by Rhodopsin in Bicelles
  • The Rhodopsin-Arrestin-1 Interaction in Bicelles
  • Detection of Structural Waters and Their Role in Structural Dynamics of Rhodopsin Activation
  • Probing Conformational Changes in Rhodopsin Using Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry
  • Analysis of Conformational Changes in Rhodopsin by Histidine Hydrogen-Deuterium Exchange
  • Investigation of Rhodopsin Dynamics in its Signaling State by Solid-State Deuterium NMR Spectroscopy
  • Sequential Structural Changes in Rhodopsin Occurring upon Photoactivation
  • Dynamic Single-Molecule Force Spectroscopy of Rhodopsin in Native Membranes
  • High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes
  • Detection of Rhodopsin Dimerization in situ by PIE-FCCS, a Time-Resolved Fluorescence Spectroscopy
  • Oligomeric State of Rhodopsin within Rhodopsin-Transducin Complex Probed with Succinylated Concanavalin A
  • Quantification of Arrestin-Rhodopsin Binding Stoichiometry
  • Rhodopsin Transient Complexes Investigated by Systems Biology Approaches
  • Three Dimensional Architecture of Murine Rod Cilium Revealed by Cryo-EM
  • Monitoring of Rhodopsin Trafficking and Mistrafficking in Live Photoreceptors
  • Measurements of Rhodopsin Diffusion within Signaling Membrane Microcompartments in Live Photoreceptors
  • Kinetics of Rhodopsin’s Chromophore Monitored in a Single Photoreceptor
  • Supplementation with Vitamin A Derivatives to Rescue Vision in Animal Models of Degenerative Retinal Diseases
  • Sustained Delivery of Retinoids to Prevent Photoreceptor Death
  • High-Throughput Screening Assays to Identify Small-Molecules Preventing Photoreceptor Degeneration Caused by the Rhodopsin P23H Mutation
  • Gene Therapy to Rescue Retinal Degeneration Caused by Mutations in Rhodopsin.