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130906s2013 xxu| s |||| 0|eng d |
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|a 9781627036375
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| 024 |
7 |
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|a 10.1007/978-1-62703-637-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Sirtuins :
|b Methods and Protocols /
|c edited by Matthew D. Hirschey.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XI, 314 p. 69 illus., 36 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1077
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| 505 |
0 |
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|a Introduction: Sirtuins in Aging and Diseases -- Sirtuins in Yeast: Phenotypes and Tools -- Studying the C. elegans Sirtuins -- Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster -- Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis -- Mass Spectrometry-Based Detection of Protein Acetylation -- SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome -- Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry -- Identification of Deacetylase Substrates with the Biotin Switch Approach -- Assaying Chromatin Sirtuins -- Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay -- Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography -- Chemical Acetylation And Deacetylation -- Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography -- In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity -- Oxygen Flux Analysis to Understand the Biological Function of Sirtuins -- The Emerging Links Between Sirtuins and Autophagy -- Methods to Study the Role of Sirtuins in Genome Stability -- Circadian Measurements of Sirtuin Biology -- Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice.
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| 650 |
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0 |
|a Animal genetics.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Animal Genetics and Genomics.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Hirschey, Matthew D.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|