Sirtuins : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
1077 |
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Table of Contents:
- Introduction: Sirtuins in Aging and Diseases
- Sirtuins in Yeast: Phenotypes and Tools
- Studying the C. elegans Sirtuins
- Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster
- Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis
- Mass Spectrometry-Based Detection of Protein Acetylation
- SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
- Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry
- Identification of Deacetylase Substrates with the Biotin Switch Approach
- Assaying Chromatin Sirtuins
- Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay
- Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography
- Chemical Acetylation And Deacetylation
- Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography
- In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
- Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
- The Emerging Links Between Sirtuins and Autophagy
- Methods to Study the Role of Sirtuins in Genome Stability
- Circadian Measurements of Sirtuin Biology
- Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice.