Type 3 Secretion Systems : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Methods in Molecular Biology,
1531 |
| Subjects: |
Table of Contents:
- Introduction to Type III Secretion Systems
- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components
- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model
- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System
- Isolation of Type III Secretion System Needle Complexes by Shearing
- Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems
- Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes
- Analysis of Type III Secretion System Secreted Proteins
- Fractionation Techniques to Examine Effector Translocation
- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies
- A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas
- Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants
- A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants
- Detecting Immune Responses to Type III Secretion Systems
- Recombinant Expression and Purification of the Shigella Translocator IpaB
- Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins
- Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins
- Identification of the Targets of Type III Secretion System Inhibitors
- Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.