Type 3 Secretion Systems : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Nilles, Matthew L. (Editor), Condry, Danielle L. Jessen. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2017.
Edition:1st ed. 2017.
Series:Methods in Molecular Biology, 1531
Subjects:
Table of Contents:
  • Introduction to Type III Secretion Systems
  • Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components
  • Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model
  • In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System
  • Isolation of Type III Secretion System Needle Complexes by Shearing
  • Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems
  • Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes
  • Analysis of Type III Secretion System Secreted Proteins
  • Fractionation Techniques to Examine Effector Translocation
  • Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies
  • A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas
  • Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants
  • A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants
  • Detecting Immune Responses to Type III Secretion Systems
  • Recombinant Expression and Purification of the Shigella Translocator IpaB
  • Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins
  • Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins
  • Identification of the Targets of Type III Secretion System Inhibitors
  • Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.