Telomeres and Telomerase : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Songyang, Zhou. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:3rd ed. 2017.
Colección:Methods in Molecular Biology, 1587
Materias:
Tabla de Contenidos:
  • Introduction to Telomeres and Telomerase
  • Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9
  • Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH)
  • Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)
  • Telomere G-Rich Overhang Length Measurement: DSN Method
  • Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA
  • Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
  • Analysis of Yeast Telomerase by Primer Extension Assays
  • Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP)
  • Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™)
  • Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques
  • Cytogenetic Analysis of Telomere Dysfunction
  • Probing the Telomere Damage Response
  • Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins
  • Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein–Protein Interactions
  • In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits
  • Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
  • Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization
  • Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP). .