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131111s2014 xxu| s |||| 0|eng d |
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|a 9781627037242
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| 024 |
7 |
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|a 10.1007/978-1-62703-724-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Complement System :
|b Methods and Protocols /
|c edited by Mihaela Gadjeva.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XVI, 383 p. 81 illus., 27 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1100
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| 505 |
0 |
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|a Overview -- Evaluation of Complement Function by ELISA -- Classical Complement Pathway Component C1q – Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q – Derivatives. Functional Characterization -- Classical Complement Pathway Components C1r and C1s – Purification from Human Serum and in Recombinant Form and Functional Characterization -- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2 -- Purification and Characterisation of human and Mouse Complement C3 -- Purification of Human Complement Protein C5 -- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry -- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin -- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing -- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System -- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins -- Purification and Functional Analysis of Human Properdin -- Purification and Functional Characterization of C4b-binding Protein (C4BP) -- Purification and Functional Characterization of Factor I -- C1 inhibitor – Quantification and Purification -- Purification, Quantification and Functional Analysis of Complement Factor H -- Factor H-related Proteins -- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS) -- Anti-Factor H Auto Antibodies Assay -- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Functional Analysis of C5a Effector Responses In Vitro and In Vivo -- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry -- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry -- Purification of C1q Receptors and Functional Analysis -- Detection of Cell Membrane-bound CD46 using Flow Cytometry -- Detection of CD59 using Flow Cytometry -- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay -- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry -- Analysis of the Complement Activation in Mice -- Opsonophagocytic Assay.
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| 650 |
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|a Immunology.
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| 650 |
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|a Antibodies.
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| 650 |
1 |
4 |
|a Immunology.
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| 650 |
2 |
4 |
|a Antibodies.
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| 700 |
1 |
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|a Gadjeva, Mihaela.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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