The Complement System : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1100 |
| Materias: |
Tabla de Contenidos:
- Overview
- Evaluation of Complement Function by ELISA
- Classical Complement Pathway Component C1q – Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q – Derivatives. Functional Characterization
- Classical Complement Pathway Components C1r and C1s – Purification from Human Serum and in Recombinant Form and Functional Characterization
- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
- Purification and Characterisation of human and Mouse Complement C3
- Purification of Human Complement Protein C5
- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
- Purification and Functional Analysis of Human Properdin
- Purification and Functional Characterization of C4b-binding Protein (C4BP)
- Purification and Functional Characterization of Factor I
- C1 inhibitor – Quantification and Purification
- Purification, Quantification and Functional Analysis of Complement Factor H
- Factor H-related Proteins
- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
- Anti-Factor H Auto Antibodies Assay
- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Functional Analysis of C5a Effector Responses In Vitro and In Vivo
- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
- Purification of C1q Receptors and Functional Analysis
- Detection of Cell Membrane-bound CD46 using Flow Cytometry
- Detection of CD59 using Flow Cytometry
- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
- Analysis of the Complement Activation in Mice
- Opsonophagocytic Assay.