Sphingosine-1-Phosphate : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Pébay, Alice. (Editor ), Turksen, Kursad. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 874
Materias:
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245 1 0 |a Sphingosine-1-Phosphate :  |b Methods and Protocols /  |c edited by Alice Pébay, Kursad Turksen. 
250 |a 1st ed. 2012. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2012. 
300 |a XI, 221 p. 39 illus., 10 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 874 
505 0 |a Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate -- Isoform-Selective Assays for Sphingosine Kinase Activity -- Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry -- A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples -- Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation -- High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes -- Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases -- Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm -- Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis -- Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System -- Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation -- Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells -- Sphingosine-1-Phosphate Signaling in Neural Progenitors -- Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis. 
650 0 |a Cell biology. 
650 0 |a Cell culture. 
650 1 4 |a Cell Biology. 
650 2 4 |a Cell Culture. 
700 1 |a Pébay, Alice.  |e editor. 
700 1 |a Turksen, Kursad.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks