Sphingosine-1-Phosphate : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Pébay, Alice. (Editor ), Turksen, Kursad. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 874
Materias:
Tabla de Contenidos:
  • Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate
  • Isoform-Selective Assays for Sphingosine Kinase Activity
  • Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry
  • A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule
  • Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples
  • Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation
  • High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes
  • Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
  • Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm
  • Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis
  • Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System
  • Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells
  • Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor
  • Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation
  • Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells
  • Sphingosine-1-Phosphate Signaling in Neural Progenitors
  • Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis.