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140303s2014 xxu| s |||| 0|eng d |
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|a 9781493903542
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| 024 |
7 |
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|a 10.1007/978-1-4939-0354-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Structural Genomics and Drug Discovery :
|b Methods and Protocols /
|c edited by Wayne F. Anderson.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XV, 344 p. 78 illus., 64 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1140
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| 505 |
0 |
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|a Data Management in the Modern Structural Biology and Biomedical Research Environment -- Structural Genomics of Human Proteins -- Target Selection for Structural Genomics of Infectious Diseases -- Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery -- High Throughput Cloning for Biophysical Applications -- Expression and Solubility Testing in a High Throughput Environment -- Protein Production for Structural Genomics Using E. coli Expression -- Eukaryotic Expression Systems for Structural Studies -- Automated Cell-free Protein Production Methods for Structural Studies -- Parallel Protein Purification -- Oxidative Refolding from Inclusion Bodies -- High throughput Crystallization Screening -- Screening Proteins for NMR Suitability -- Salvage or Recovery of Failed Targets by in situ Proteolysis -- Salvage of Failed Protein Targets by Reductive Alkylation -- Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy -- Data Collection for Crystallographic Structure Determination -- Structure, Determination, Refinement, and Validation -- Virtual High-Throughput Ligand Screening -- Ligand Screening using Fluorescence Thermal Shift Analysis (FTS) -- Ligand Screening using Enzymatic Assays -- Ligand Screening using NMR -- Screening Ligands by X-ray Crystallography -- Case Study – Structural Genomics and Human Protein Kinases.
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| 650 |
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|a Pharmacology.
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| 650 |
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|a Human genetics.
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| 650 |
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4 |
|a Pharmacology/Toxicology.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Anderson, Wayne F.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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