Structural Genomics and Drug Discovery : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1140 |
| Materias: |
Tabla de Contenidos:
- Data Management in the Modern Structural Biology and Biomedical Research Environment
- Structural Genomics of Human Proteins
- Target Selection for Structural Genomics of Infectious Diseases
- Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
- High Throughput Cloning for Biophysical Applications
- Expression and Solubility Testing in a High Throughput Environment
- Protein Production for Structural Genomics Using E. coli Expression
- Eukaryotic Expression Systems for Structural Studies
- Automated Cell-free Protein Production Methods for Structural Studies
- Parallel Protein Purification
- Oxidative Refolding from Inclusion Bodies
- High throughput Crystallization Screening
- Screening Proteins for NMR Suitability
- Salvage or Recovery of Failed Targets by in situ Proteolysis
- Salvage of Failed Protein Targets by Reductive Alkylation
- Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
- Data Collection for Crystallographic Structure Determination
- Structure, Determination, Refinement, and Validation
- Virtual High-Throughput Ligand Screening
- Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
- Ligand Screening using Enzymatic Assays
- Ligand Screening using NMR
- Screening Ligands by X-ray Crystallography
- Case Study – Structural Genomics and Human Protein Kinases.