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110825s2011 xxu| s |||| 0|eng d |
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|a 9781617791604
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024 |
7 |
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|a 10.1007/978-1-61779-160-4
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Signal Transduction Protocols /
|c edited by Louis M. Luttrell, Stephen S. G. Ferguson.
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250 |
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|a 3rd ed. 2011.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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300 |
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|a XIV, 430 p. 89 illus., 10 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 756
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505 |
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|a Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling -- Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes -- Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis -- RGS-insensitive Ga Subunits: Probes of Ga Subtype-selective Signaling and Physiological Functions of RGS Proteins -- Bioinformatic Approaches to Metabolic Pathways Analysis -- Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET -- Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes -- Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL) -- Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization -- Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization -- Analysis of GPCR/Ion Channel Interactions -- Multicolor BiFC Analysis of G Protein bg Complex Formation and Localization -- Real-Time BRET Assays to Measure G Protein/Effector Interactions -- Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP -- Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters -- Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity -- Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells -- Visualizing Receptor Endocytosis and Trafficking -- Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM -- Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy -- Detection and Characterization of Receptor Interactions with PDZ Domains -- Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins -- Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP -- Disrupting Protein Complexes Using Tat-tagged Peptide Mimics -- Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells.
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650 |
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|a Cytology.
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650 |
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|a RNA-ligand interactions.
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650 |
1 |
4 |
|a Cell Biology.
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650 |
2 |
4 |
|a Protein-Ligand Interactions.
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700 |
1 |
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|a Luttrell, Louis M.
|e editor.
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700 |
1 |
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|a Ferguson, Stephen S. G.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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