Signal Transduction Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Luttrell, Louis M. (Editor ), Ferguson, Stephen S. G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:3rd ed. 2011.
Colección:Methods in Molecular Biology, 756
Materias:
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020 |a 9781617791604 
024 7 |a 10.1007/978-1-61779-160-4  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Signal Transduction Protocols /  |c edited by Louis M. Luttrell, Stephen S. G. Ferguson. 
250 |a 3rd ed. 2011. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2011. 
300 |a XIV, 430 p. 89 illus., 10 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 756 
505 0 |a Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling -- Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes -- Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis -- RGS-insensitive Ga Subunits: Probes of Ga Subtype-selective Signaling and Physiological Functions of RGS Proteins -- Bioinformatic Approaches to Metabolic Pathways Analysis -- Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET -- Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes -- Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL) -- Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization -- Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization -- Analysis of GPCR/Ion Channel Interactions -- Multicolor BiFC Analysis of G Protein bg Complex Formation and Localization -- Real-Time BRET Assays to Measure G Protein/Effector Interactions -- Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP -- Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters -- Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity -- Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells -- Visualizing Receptor Endocytosis and Trafficking -- Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM -- Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy -- Detection and Characterization of Receptor Interactions with PDZ Domains -- Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins -- Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP -- Disrupting Protein Complexes Using Tat-tagged Peptide Mimics -- Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells. 
650 0 |a Cytology. 
650 0 |a RNA-ligand interactions. 
650 1 4 |a Cell Biology. 
650 2 4 |a Protein-Ligand Interactions. 
700 1 |a Luttrell, Louis M.  |e editor. 
700 1 |a Ferguson, Stephen S. G.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks