Signal Transduction Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Luttrell, Louis M. (Editor ), Ferguson, Stephen S. G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:3rd ed. 2011.
Colección:Methods in Molecular Biology, 756
Materias:
Tabla de Contenidos:
  • Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
  • Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes
  • Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
  • RGS-insensitive Ga Subunits: Probes of Ga Subtype-selective Signaling and Physiological Functions of RGS Proteins
  • Bioinformatic Approaches to Metabolic Pathways Analysis
  • Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
  • Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes
  • Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL)
  • Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization
  • Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization
  • Analysis of GPCR/Ion Channel Interactions
  • Multicolor BiFC Analysis of G Protein bg Complex Formation and Localization
  • Real-Time BRET Assays to Measure G Protein/Effector Interactions
  • Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
  • Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
  • Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
  • Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
  • Visualizing Receptor Endocytosis and Trafficking
  • Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM
  • Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy
  • Detection and Characterization of Receptor Interactions with PDZ Domains
  • Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins
  • Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP
  • Disrupting Protein Complexes Using Tat-tagged Peptide Mimics
  • Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells.