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02253nam a22003135i 4500 |
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130830s2013 xxu| s |||| 0|eng d |
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|a 9781627036252
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| 024 |
7 |
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|a 10.1007/978-1-62703-625-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Synthetic Biology /
|c edited by Karen M. Polizzi, Cleo Kontoravdi.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a X, 226 p. 80 illus., 48 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1073
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| 505 |
0 |
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|a What is Synthetic Biology? -- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program -- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit -- Overlap Extension PCR Cloning -- One-Step Isothermal Assembly of DNA Fragments -- Creation and Characterization of Component Libraries for Synthetic Biology -- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements -- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli -- Construction and Engineering of Large Biochemical Pathways via DNA Assembler -- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors -- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research -- Combinatorial DNA Assembly using Golden Gate Cloning -- Construction of Synthetic Gene Circuits in the Escherichia coli Genome -- Shuffling of DNA Cassettes in a Synthetic Integrin -- Systematic Methodology for the Development of Mathematical Models for Biological Processes -- SOBOLHDMR: A General Purpose Modelling Software.
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| 650 |
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|a Biotechnology.
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| 650 |
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|a Biochemical engineering.
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| 650 |
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4 |
|a Biotechnology.
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| 650 |
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4 |
|a Biochemical Engineering.
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| 700 |
1 |
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|a Polizzi, Karen M.
|e editor.
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| 700 |
1 |
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|a Kontoravdi, Cleo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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