Synthetic Biology /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
1073 |
| Materias: |
Tabla de Contenidos:
- What is Synthetic Biology?
- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
- Overlap Extension PCR Cloning
- One-Step Isothermal Assembly of DNA Fragments
- Creation and Characterization of Component Libraries for Synthetic Biology
- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements
- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
- Construction and Engineering of Large Biochemical Pathways via DNA Assembler
- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors
- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
- Combinatorial DNA Assembly using Golden Gate Cloning
- Construction of Synthetic Gene Circuits in the Escherichia coli Genome
- Shuffling of DNA Cassettes in a Synthetic Integrin
- Systematic Methodology for the Development of Mathematical Models for Biological Processes
- SOBOLHDMR: A General Purpose Modelling Software.