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161109s2016 gw | s |||| 0|eng d |
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|a 9783319422268
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| 024 |
7 |
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|a 10.1007/978-3-319-42226-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Rolling Circle Amplification (RCA) :
|b Toward New Clinical Diagnostics and Therapeutics /
|c edited by Vadim V. Demidov.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a Cham :
|b Springer International Publishing :
|b Imprint: Springer,
|c 2016.
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| 300 |
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|a XVII, 176 p. 53 illus., 37 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|a Preface -- Part 1. Improved DNA polymerases and new DNA and DNA/RNA ligases useful in RCA -- 1. Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs -- 2. Preparation of circular templates by T4 RNA ligase 2 for rolling circle amplification of target microRNAs with high specificity and sensitivity -- 3. Use of DNA CircLigase for direct isothermal detection of microbial mRNAs by RNA-primed rolling circle amplification and preparation of ø29 DNA polymerase not contaminated -- Part 2. RCA-involving techniques with enhanced signal amplification -- 4. Nicking enzyme-assisted branched-chain RCA reaction for cascade DNA amplification -- 5. Combining isothermal amplification techniques: Coupled RCA-LAMP -- Part 3. Emerging RCA diagnostics -- 6. Detection of vascular disease-related single nucleotide polymorphisms in clinical samples using ramified rolling circle amplification -- 7. Ultrasensitive isothermal detection of protein analytes using rolling circle amplification in micro-scale platforms -- 8. Rolling circle amplification with padlock probes for in situ detection of RNA analytes -- 9. PNA-assisted rolling circle amplification for detection of DNA marker sequences in human cells -- 10. Sensor systems with magnetic and optomagnetic readout of rolling circle amplification products -- Part 4. Prospective RCA-based therapeutics -- 11. DNA nanoclews for stimuli-responsive anticancer drug delivery -- 12. RCA-assisted self-assembled DNA origami nano-constructs as vehicles for cellular delivery of diagnostic probes and therapeutic drugs -- 13. DeNAno: a novel multivalent affinity reagent produced by selection of RCA-generated DNA nanoparticle libraries -- Index.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
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0 |
|a Biochemical engineering.
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| 650 |
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|a Medical biochemistry.
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| 650 |
1 |
4 |
|a Enzymology.
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| 650 |
2 |
4 |
|a Biochemical Engineering.
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| 650 |
2 |
4 |
|a Medical Biochemistry.
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| 700 |
1 |
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|a Demidov, Vadim V.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|