Rolling Circle Amplification (RCA) : Toward New Clinical Diagnostics and Therapeutics /
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| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Cham :
Springer International Publishing : Imprint: Springer,
2016.
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| Edición: | 1st ed. 2016. |
| Materias: |
Tabla de Contenidos:
- Preface
- Part 1. Improved DNA polymerases and new DNA and DNA/RNA ligases useful in RCA
- 1. Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs
- 2. Preparation of circular templates by T4 RNA ligase 2 for rolling circle amplification of target microRNAs with high specificity and sensitivity
- 3. Use of DNA CircLigase for direct isothermal detection of microbial mRNAs by RNA-primed rolling circle amplification and preparation of ø29 DNA polymerase not contaminated
- Part 2. RCA-involving techniques with enhanced signal amplification
- 4. Nicking enzyme-assisted branched-chain RCA reaction for cascade DNA amplification
- 5. Combining isothermal amplification techniques: Coupled RCA-LAMP
- Part 3. Emerging RCA diagnostics
- 6. Detection of vascular disease-related single nucleotide polymorphisms in clinical samples using ramified rolling circle amplification
- 7. Ultrasensitive isothermal detection of protein analytes using rolling circle amplification in micro-scale platforms
- 8. Rolling circle amplification with padlock probes for in situ detection of RNA analytes
- 9. PNA-assisted rolling circle amplification for detection of DNA marker sequences in human cells
- 10. Sensor systems with magnetic and optomagnetic readout of rolling circle amplification products
- Part 4. Prospective RCA-based therapeutics
- 11. DNA nanoclews for stimuli-responsive anticancer drug delivery
- 12. RCA-assisted self-assembled DNA origami nano-constructs as vehicles for cellular delivery of diagnostic probes and therapeutic drugs
- 13. DeNAno: a novel multivalent affinity reagent produced by selection of RCA-generated DNA nanoparticle libraries
- Index.