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161027s2017 xxu| s |||| 0|eng d |
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|a 9781493964482
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| 024 |
7 |
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|a 10.1007/978-1-4939-6448-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Semaphorin Signaling :
|b Methods and Protocols /
|c edited by Jonathan R. Terman.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XV, 503 p. 96 illus., 54 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1493
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| 505 |
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|a Semaphorins and their Signaling Mechanisms -- Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells -- Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction -- Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression Systems -- Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions -- Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry -- In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin -- Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component -- Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins -- Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins -- Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components -- Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition -- Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse -- An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse -- Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins – A Primary Culture Approach -- Characterizing Semaphorin Signaling Using Isolated Neurons in Culture -- Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues -- Assays to Examine Transmembrane Semaphorin Function In Vitro -- Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth -- Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins -- Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth -- Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones -- Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors -- Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development -- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling -- Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells -- Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay -- Podocyte Shape Regulation by Semaphorin 3A and MICAL-1 -- In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling -- Assaying the Action of Secreted Semaphorins on Vascular Permeability -- Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis -- Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development -- Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models -- Characterizing Semaphorin Signaling In Vivo Using C. Elegans. .
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Terman, Jonathan R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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