Semaphorin Signaling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1493 |
| Materias: |
Tabla de Contenidos:
- Semaphorins and their Signaling Mechanisms
- Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells
- Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction
- Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression Systems
- Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions
- Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry
- In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin
- Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component
- Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins
- Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins
- Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components
- Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition
- Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse
- An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse
- Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins – A Primary Culture Approach
- Characterizing Semaphorin Signaling Using Isolated Neurons in Culture
- Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues
- Assays to Examine Transmembrane Semaphorin Function In Vitro
- Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth
- Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins
- Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth
- Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones
- Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors
- Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development
- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling
- Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells
- Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay
- Podocyte Shape Regulation by Semaphorin 3A and MICAL-1
- In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling
- Assaying the Action of Secreted Semaphorins on Vascular Permeability
- Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis
- Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development
- Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models
- Characterizing Semaphorin Signaling In Vivo Using C. Elegans. .