Schizosaccharomyces pombe : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Singleton, Teresa L. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2018.
Edition:1st ed. 2018.
Series:Methods in Molecular Biology, 1721
Subjects:
Table of Contents:
  • Preparation of Solutions and Reagents
  • Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing
  • Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
  • Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
  • Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
  • Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
  • Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis
  • The No-nonsens SDS-PAGE
  • Crystallization of Recombinant α-actinin and Related Proteins
  • Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy
  • Antibody Pull-down Experiments in Fission Yeast
  • Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
  • Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
  • In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe
  • High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast
  • Random Spore Analysis in Fission Yeast
  • Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
  • Schizosaccharomyces pombe Biotechnological Applications in Winemaking
  • Schizosaccharomyces pombe Isolation Protocol. .