Zinc Finger Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1867 |
| Materias: |
Tabla de Contenidos:
- Part 1. Basic Biology and Engineering of ZFPs
- PRDM14, a zinc finger protein, regulates cancer stemness
- Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins
- Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation
- Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology
- Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers
- Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins
- Directed evolution of targeted recombinases for genome engineering
- Optical recording of cellular zinc dynamics with zinc finger based biosensors
- Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins
- Genome editing of MSCs as a platform for cell therapy
- Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity
- Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells
- Selection and characterization of DNA aptamers against FokI nuclease domain
- An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs
- Part 3. Delivery Methods of ZFPs
- Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering
- Gene editing in channel catfish via double electroporation of zinc finger nucleases
- Delivery of mtZFNs into early mouse embryos
- Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain
- In-vivo applications of cell-penetrating zinc finger transcription factors
- Manufacturing and delivering genome-editing proteins.