Viral Vectors for Gene Therapy : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Manfredsson, Fredric P. (Editor ), Benskey, Matthew J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1937
Materias:
Tabla de Contenidos:
  • Basic Concepts in Viral Vector-Mediated Gene Therapy
  • Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing
  • Design, Construction, and Application of Transcription Activation-Like Effectors
  • Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain
  • AAV Production using Baculovirus Expression Vector System
  • Multimodal Production of Adeno-Associated Virus
  • Generation of High Titer Pseudotyped Lentiviral Particles
  • A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration
  • Current Use of Adenovirus Vectors and their Purification Methods
  • Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest
  • Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes
  • AAV Mediated Gene Delivery to the Mouse Liver
  • Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse
  • Gene Transfer to Mouse Kidney In Vivo
  • Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders
  • Localized Intra-Arterial Gene Delivery using AAV
  • Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors
  • Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs
  • Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons
  • Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice
  • Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates.